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KMID : 0613820050150050802
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Journal of Life Science
2005 Volume.15 No. 5 p.802 ~ p.808
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Silencing of Disabled-2 Gene by CpG Methylation in Human Breast Cancer Cell Line, MDA MB-231 Cells
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Ko Myung-Hyun
Oh Yu-Mi
Park Jun-Ho
Jeon Byung-Hoon
Han Dong-Min
Kim Won-Sin
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Abstract
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Human Disabled-2 (Dab2) is a candidate tumor suppressor gene that regulates cell growth by c-Fos suppression in normal cells. In many cancer cells, Dab2 expression is lost or greatly diminished in ～85% of the breast and ovarian cancers. In this study, we have examined the methylation status of CpG island on Dab2 gene promoter using bisulfite-assisted genomic sequencing and methylation specific PCR (MSP) method in human breast cancer cell line, MDA MB-231 cells. In normal human uterus endometrial cells, Dab2 was completely unmethylated. In contrast, Dab2 was methylated on CpG dinucleotides near the TATA box in MDA MB-231 cells. Following MDA MB-231 cells by treatment with 5-azacytidine, Dab2 gene were demethylated and reexpressed. Result of this study suggested that silencing of Dab2 gene is correlated to CpG island methylation in human breast cancer cell line, MBA MD-231 cells.
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